Department of Biology, Yale University, P.O. Box 6666, 06511, New Haven, CT, USA.
Plant Cell Rep. 1983 Jun;2(3):119-21. doi: 10.1007/BF00269333.
High yields of mesophyll protoplasts were obtained after treatment of leaves of Trigonella foenumgraecum, a forage legume, with purified cellulase. Under appropriate conditions of culture up to 70% plating efficiency could be obtained. The protoplast-derived colonies developed into rapidly growing green calli and produced leafy shoots on a medium containing 0.1 mg/l each of benzylaminopurine (BAP) and zeatin. Addition of glutamine and asparagine to the medium was found essential for rapid cell division, callus growth and differentiation.
经过纯化的纤维素酶处理，饲料豆科植物胡芦巴的叶片可以获得高产的叶肉原生质体。在适当的培养条件下，可达 70%的平板效率。由原生质体衍生的菌落发育成快速生长的绿色愈伤组织，并在含有 0.1mg/L 苄基氨基嘌呤（BAP）和玉米素的培养基上产生具叶的芽。发现向培养基中添加谷氨酰胺和天冬酰胺对于快速细胞分裂、愈伤组织生长和分化是必需的。
