Pendy L M, Arra S J, Anselmino L M, Thomas L L
J Immunol Methods. 1986 Jul 11;91(1):59-63. doi: 10.1016/0022-1759(86)90102-x.
A flow cytometric procedure is described for the isolation of human basophils. The basophils are isolated by cell sorting after passive sensitization with mouse FITC-IgE anti-DNP, and the isolated basophils exhibit characteristic histamine release in response to incubation with DNP21-human serum albumin conjugates. Using this method, 1-4 X 10(5) basophils can be isolated at greater than or equal to 98% purity from approximately 30 ml whole blood in a 2 h sorting procedure.
本文描述了一种用于分离人嗜碱性粒细胞的流式细胞术方法。用小鼠FITC-IgE抗DNP进行被动致敏后，通过细胞分选分离嗜碱性粒细胞，分离出的嗜碱性粒细胞在与DNP21-人血清白蛋白缀合物孵育时表现出特征性的组胺释放。使用该方法，在2小时的分选过程中，可从约30毫升全血中以大于或等于98%的纯度分离出1 - 4×10⁵个嗜碱性粒细胞。
