Weil G J, Leiserson W M, Chused T M
J Immunol Methods. 1983 Mar 25;58(3):359-63. doi: 10.1016/0022-1759(83)90363-0.
Human mononuclear cells labeled with fluorescein-conjugated anti-IgE antibody were subjected to flow microfluorometric analysis. Basophils were enriched to 97-99% purity with a 2-step cell sorting procedure. Trypan blue exclusion of sorted cells exceeded 90% and the net yield of the procedure was 15%.
用荧光素偶联的抗IgE抗体标记的人单核细胞进行流式微量荧光分析。通过两步细胞分选程序将嗜碱性粒细胞富集至纯度为97 - 99%。分选细胞的台盼蓝排斥率超过90%，该程序的净产量为15%。
