两种伊朗蝰蛇（锯鳞蝰和波斯角蝰费氏亚种）毒液的部分分离及其抗血小板活性评估

Mehdizadeh Kashani Toktam, Vatanpour Hossein, Zolfagharian Hossein, Hooshdar Tehrani Hasan, Heydari Mohammad Hossein, Kobarfard Farzad

Department of Toxicology, School of Pharmacy, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

Iran J Pharm Res. 2012 Fall;11(4):1183-9.

Platelet aggregation inhibitory effect and anticoagulant properties of fractions separated from the venoms of Cerastes persicus fieldi and Echis carinatus were investigated. The partial fractionation was performed on a Sephadex G-100 column. Two fractions separated from Cerastes persicus fieldi showed anti platelet aggregation activity on ADP (200 μM)-induced platelet aggregation (ca 80% inhibition). Attempts to measure the antiplatelet aggregation activity of crude Echis carinatus venom and its fractions were not successful due to the protein coagulation of the plasma samples after the addition of venom. Anticoagulant activities of venoms were also evaluated. Total venom of Echis carinatus showed anti coagulant activity in PT test, while its fractions showed procoagulant activity.

研究了波斯锯鳞蝰（Cerastes persicus fieldi）和锯鳞蝰（Echis carinatus）毒液分离组分的血小板聚集抑制作用和抗凝特性。在葡聚糖凝胶G - 100柱上进行了部分分级分离。从波斯锯鳞蝰分离出的两个组分对ADP（200μM）诱导的血小板聚集表现出抗血小板聚集活性（约80%抑制）。由于加入毒液后血浆样本发生蛋白质凝固，未能成功测量锯鳞蝰粗毒液及其组分的抗血小板聚集活性。还评估了毒液的抗凝活性。锯鳞蝰的总毒液在PT试验中表现出抗凝活性，而其组分表现出促凝活性。