Taherianfard Mahnaz, Sharifi Maryam, Tadjali Mina, Kohkiloezadeh Mahboubeh
Associate Professor, Department of Physiology, School of Veterinary Medicine, Shiraz University, Shiraz, Iran.
Iran J Neurol. 2012;11(4):140-5.
Learning and memory are the most intensively studied subjects in neuroscience. Two sites of mammalian brain which are important in learning and memory are CA1 region of hippocampus and Purkinje cell layer of cerebellum. So, the aim of present investigation was to study of the effect of ovariectomy and passive avoidance learning on NR1 subunit of N-methyl-D-aspartate (NMDA) receptor distribution in CA1 region of hippocampus and Purkinje cell layer of cerebellum.
Twenty four Sprague-Dawley rats were used in 4 groups: control-1 (intact without learning), control-2 (intact with learning), ovariectomy without learning, and ovariectomy with learning. Immunohistochemical procedure was used for determination of NR1 subunit of NMDA receptor. A shuttle box apparatus used for passive avoidance learning procedure. The determination of color intensity was cone by Photoshop software.
Immunohistological findings indicated that ovariectomy has a negative effect on density of NR1 subunit of NMDA receptors in two brain regions. Passive avoidance learning significantly increased density of NR1 subunit of NMDA receptors in two brain regions.
The results indicated that the sex hormone can modulate function and expression of the NR1 subunit of NMDA receptor in CA1 region of hippocampus and Purkinje cell layer of cerebellum.
学习与记忆是神经科学领域研究最为深入的课题。哺乳动物大脑中对学习与记忆至关重要的两个部位是海马体的CA1区和小脑的浦肯野细胞层。因此，本研究的目的是探讨卵巢切除术及被动回避学习对海马体CA1区和小脑浦肯野细胞层中N-甲基-D-天冬氨酸（NMDA）受体NR1亚基分布的影响。
选用24只Sprague-Dawley大鼠，分为4组：对照组1（完整未学习）、对照组2（完整已学习）、未学习的卵巢切除组和已学习的卵巢切除组。采用免疫组织化学方法测定NMDA受体的NR1亚基。使用穿梭箱装置进行被动回避学习实验。通过Photoshop软件测定颜色强度。
免疫组织学结果表明，卵巢切除术对两个脑区中NMDA受体NR1亚基的密度有负面影响。被动回避学习显著增加了两个脑区中NMDA受体NR1亚基的密度。
结果表明，性激素可调节海马体CA1区和小脑浦肯野细胞层中NMDA受体NR1亚基的功能和表达。
