Maudelonde T, Rosenfield R L, Shuler C F, Schwartz S A
J Steroid Biochem. 1986 May;24(5):1053-60. doi: 10.1016/0022-4731(86)90359-6.
In order to study the mechanism of action of androgen on pubic and scalp hair, we established these and skin epithelial cells in culture. Because 5 alpha-reductase has been suspected of playing a role in hair growth, we tested the possibility that these cells differ in their pattern of androgen metabolism. Furthermore, we tested the hypothesis that androgen exerts its distinctive effects on these hairs by differentially regulating keratin or DNA synthesis. Anagen hairs of men and women were plucked from the pubis or scalp vertex and were studied using an epithelial cell culture technique. DHT formation from [3H]T cultured skin cells increased in the following order: epidermal less than scalp less than pubic less than fibroblasts = 0.8:2.8:8.1:71%/mg DNA/min, respectively. Androstanediols were minor [3H]DHT metabolites of all these skin cell types. The only feature that distinguished among the cultured epithelial cells was the ratio of apparent 5 alpha-reductase (5 alpha-R) to 17 beta-hydroxysteroid dehydrogenase (17 beta-HSD) activity: this was significantly greater (P less than 0.05) in cultured pubic hair cells than in scalp hair or epidermal cells. Cultured scalp and pubic hair cells resembled freshly plucked hair follicle cells in their keratin pattern. 46, 50, 56 and 58 kdalton bands constituted 99% of the total keratins. This keratin pattern and the polygonal cell shape were also similar to that of cultured epidermal cells. However, this keratin pattern was distinctly different from that of hair shafts which have 53 and 63 kdalton keratins. Dihydrotestosterone did not affect the keratin pattern, pattern of incorporation of [35S]cysteine or [35S]methionine, or rates of protein synthesis or cell proliferation in cultured hair cells. Although the higher apparent 5 alpha-R/17 beta-HSD ratio of cultured pubic than of scalp hairs is compatible with modulation of hair development by androgen, these studies militate against the possibility that androgens directly affect hair cell proliferation or protein synthesis in pubic or scalp hair.
为了研究雄激素对阴毛和头皮毛发的作用机制，我们在培养中建立了这些毛发和皮肤上皮细胞。由于怀疑5α-还原酶在头发生长中起作用，我们测试了这些细胞在雄激素代谢模式上是否存在差异的可能性。此外，我们还测试了以下假设：雄激素通过差异调节角蛋白或DNA合成，对这些毛发发挥其独特作用。从男性和女性的耻骨或头皮顶部拔取生长期毛发，并使用上皮细胞培养技术进行研究。培养的皮肤细胞中由[3H]睾酮形成双氢睾酮（DHT）的量按以下顺序增加：表皮细胞＜头皮细胞＜耻骨细胞＜成纤维细胞，分别为0.8:2.8:8.1:71%/mg DNA/分钟。雄甾二醇是所有这些皮肤细胞类型中次要的[3H]DHT代谢产物。培养的上皮细胞之间唯一的区别特征是表观5α-还原酶（5α-R）与17β-羟基类固醇脱氢酶（17β-HSD）活性的比值：培养的阴毛细胞中的这一比值显著高于头皮毛发或表皮细胞（P＜0.05）。培养的头皮和阴毛细胞在角蛋白模式上类似于刚拔下的毛囊细胞。46、50、56和58千道尔顿的条带占总角蛋白的99%。这种角蛋白模式和多边形细胞形状也与培养的表皮细胞相似。然而，这种角蛋白模式与具有53和63千道尔顿角蛋白的毛干明显不同。双氢睾酮不影响培养的毛发细胞中的角蛋白模式、[35S]半胱氨酸或[35S]甲硫氨酸的掺入模式、蛋白质合成速率或细胞增殖。尽管培养的阴毛中表观5α-R/17β-HSD比值高于头皮毛发，这与雄激素调节毛发发育一致，但这些研究排除了雄激素直接影响阴毛或头皮毛发细胞增殖或蛋白质合成的可能性。
