合成的α-(1→3)分支D-葡聚糖与葡聚糖B1375的免疫化学相似性。

Immunochemical similarity of synthetic alpha-(1----3)-branched D-glucans to dextran B1375.

作者信息

Torii M, Yamazoe M, Ogawa S, Watabe K, Koshikawa T, Uryu T, Schuerch C

出版信息

Microbiol Immunol. 1986;30(3):261-8. doi: 10.1111/j.1348-0421.1986.tb00941.x.

DOI:10.1111/j.1348-0421.1986.tb00941.x

Abstract

Anti-dextran B1375 antibodies were raised in rabbits by injecting formalin-killed Leuconostoc mesenteroides strain NRRL B1375, and the anti-dextran serum was used to examine native dextran B1375, and synthetic linear and four alpha-(1----3)-branched alpha-(1----6)-D-glucopyranans for similarities. The antiserum reacted with the homologous dextran B1375 and also with all the synthetic linear and branched glucans. Precipitation and precipitation-inhibition studies indicated that the antiserum contained at least three groups of antibodies with different specificities, the first specific for linear alpha-(1----6)-D-glucopyranan structure, the second specific for alpha-D-glycopyranosyl-(1----3)-branching and the last specific for another, unknown structure present in the dextran B1375 molecule. Two samples of the synthetic branched glucans were shown to be immunochemically the most similar to natural dextran B1375 by inhibition experiments.

摘要

通过注射福尔马林灭活的肠系膜明串珠菌NRRL B1375菌株，在兔体内产生了抗葡聚糖B1375抗体，并使用该抗葡聚糖血清检测天然葡聚糖B1375以及合成的线性和四种α-(1→3)-分支的α-(1→6)-D-吡喃葡萄糖聚糖的相似性。抗血清与同源葡聚糖B1375以及所有合成的线性和分支葡聚糖发生反应。沉淀和沉淀抑制研究表明，抗血清至少包含三组具有不同特异性的抗体，第一组对线性α-(1→6)-D-吡喃葡萄糖聚糖结构具有特异性，第二组对α-D-吡喃葡萄糖基-(1→3)-分支具有特异性，最后一组对葡聚糖B1375分子中存在的另一种未知结构具有特异性。通过抑制实验表明，两种合成分支葡聚糖样品在免疫化学上与天然葡聚糖B1375最为相似。