葡聚糖的免疫化学研究。与兔抗葡聚糖B1355的两种不同的α1连接至3种特异性。

Immunochemical studies on dextrans. Two distinct alpha 1 linked to 3 specificities of rabbit anti-dextran B1355.

作者信息

Torii M, Alberto B P, Tanaka S

出版信息

J Biochem. 1980 Dec;88(6):1855-9. doi: 10.1093/oxfordjournals.jbchem.a133161.

DOI:10.1093/oxfordjournals.jbchem.a133161

PMID:6161919

Abstract

Rabbit anti-dextran B1355 sera prepared by injecting rabbits with Leuconostoc mesenteroides NRRL B1355 were separated on a Sephadex G75 column into two fractions, one binding and the other not binding to the column. Oligosaccharide inhibition of precipitation of the two fractions with dextran B1355 indicated that both fractions had alpha 1 linked to 3 specificity. However, antibodies in the non-binding fraction were shown to be directed against O-alpha-D-glucopyranosyl-(1 linked to 3)-O-alpha-D-glucopyranosyl-(1 linked to 6)-D-glucose, while those in the binding fraction were directed against O-alpha-D-glucopyranosyl-(1 linked to 6)-O-alpha-D-glucopyranosyl-(1 linked to 3)-D-glucose. These results are consistent with the proposal of Bhoopalam et al. (Proc. Soc. Exp. Biol. Med. (1979(=) 161, 430-434) that there are different epitopic groups on this dextran.

摘要

通过向兔子注射肠系膜明串珠菌NRRL B1355制备的兔抗葡聚糖B1355血清在葡聚糖G75柱上分离成两个组分，一个与柱结合，另一个不与柱结合。用葡聚糖B1355对这两个组分的沉淀进行寡糖抑制表明，两个组分都具有α1连接到3的特异性。然而，未结合组分中的抗体显示针对O-α-D-吡喃葡萄糖基-（1连接到3）-O-α-D-吡喃葡萄糖基-（1连接到6）-D-葡萄糖，而结合组分中的抗体则针对O-α-D-吡喃葡萄糖基-（1连接到6）-O-α-D-吡喃葡萄糖基-（1连接到3）-D-葡萄糖。这些结果与Bhoopalam等人（《实验生物学与医学学会会刊》（1979年（=）161, 430 - 434））的提议一致，即在这种葡聚糖上存在不同的表位基团。