Department of Environmental Engineering, Seoul National University of Science and Technology, Seoul, Republic of Korea.
Department of Industrial Health, Catholic University of Pusan, Busan, Republic of Korea.
Waste Manag. 2014 Jun;34(6):1006-11. doi: 10.1016/j.wasman.2013.10.039. Epub 2013 Nov 16.
Swine manure was subjected to laboratory scale composting in order to quantify bioaerosols, i.e., airborne culturable bacteria and endotoxin, in the exhaust gas, which provided details on the effect of temperature on bacterial emissions. The concentration of airborne bacteria reached 31,250 colony-forming units (CFU)/m(3) during the thermophilic stage of composting, and positively correlated with the temperature profile of the compost pile. Initially, the endotoxin concentration was 1820 endotoxin units (EU)/m(3), but it decreased exponentially as the composting process proceeded. The temperature can be an excellent indicator of bacterial emissions during the composting process, indicating that the composting process requires a consistently high temperature to ensure sanitization of both compost and bacterial emissions. The cumulative emission data showed that emission factors was 11.2-13.5 CFU/g dry swine manure and that of endotoxin was 0.5-0.9 EU/g dry swine manure. The bacterial diversity in the bioaerosol was analyzed by polymerase chain reaction-denaturing gradient gel electrophoresis, revealing the presence of various gram-negative bacterial consortia.
为了量化堆肥废气中的生物气溶胶（即空气中可培养细菌和内毒素），对猪粪进行了实验室规模的堆肥，详细说明了温度对细菌排放的影响。在堆肥的高温阶段，空气中细菌的浓度达到了 31250 个菌落形成单位（CFU）/m³，与堆肥堆的温度分布呈正相关。最初，内毒素浓度为 1820 个内毒素单位（EU）/m³，但随着堆肥过程的进行，内毒素浓度呈指数下降。温度可以是堆肥过程中细菌排放的极好指标，表明堆肥过程需要保持一致的高温，以确保堆肥和细菌排放的消毒。累积排放数据表明，排放因子为 11.2-13.5 CFU/g 干猪粪，内毒素排放因子为 0.5-0.9 EU/g 干猪粪。通过聚合酶链反应-变性梯度凝胶电泳分析了生物气溶胶中的细菌多样性，结果表明存在各种革兰氏阴性细菌群落。
