Sino-Forest Applied Research Centre for Pearl River Delta Environment, Hong Kong Baptist University, Kowloon Tong, Hong Kong.
Bioresour Technol. 2012 Dec;126:383-90. doi: 10.1016/j.biortech.2012.03.045. Epub 2012 Mar 20.
This study monitored the abundance of antibiotic resistant genes (ARGs) and the bacterial diversity during composting of swine manure spiked with chlortetracycline, sulfadiazine and ciprofloxacin at two different levels and a control without antibiotics. Resistance genes of tetracycline (tetQ, tetW, tetC, tetG, tetZ and tetY), sulfonamide (sul1, sul2, dfrA1 and dfrA7) and fluoroquinolone (gyrA and parC) represented 0.02-1.91%, 0.67-10.28% and 0.00005-0.0002%, respectively, of the total 16S rDNA copies in the initial composting mass. After 28-42 days of composting, these ARGs, except parC, were undetectable in the composting mass indicating that composting is a potential method of manure management. Polymerase chain reaction-denaturing gradient gel electrophoresis analysis of bacterial 16S rDNA of the composting mass indicated that the addition of antibiotics up to 100, 20 and 20mg/kg of chlortetracycline, sulfadiazine and ciprofloxacin, respectively, elicited only a transient perturbation and the bacterial diversity was restored in due course of composting.
本研究监测了添加不同浓度（100、20 和 20mg/kg）金霉素、磺胺嘧啶和环丙沙星的猪粪在堆肥过程中抗生素耐药基因（ARGs）的丰度和细菌多样性，以及未添加抗生素的对照处理。四环素（tetQ、tetW、tetC、tetG、tetZ 和 tetY）、磺胺类（sul1、sul2、dfrA1 和 dfrA7）和氟喹诺酮类（gyrA 和 parC）耐药基因分别占初始堆肥质量中总 16S rDNA 拷贝数的 0.02-1.91%、0.67-10.28%和 0.00005-0.0002%。28-42 天后，堆肥中除 parC 外，这些 ARGs 均无法检测到，表明堆肥是一种潜在的粪便管理方法。对堆肥中细菌 16S rDNA 的聚合酶链反应-变性梯度凝胶电泳分析表明，添加高达 100、20 和 20mg/kg 的金霉素、磺胺嘧啶和环丙沙星仅引起短暂的扰动，细菌多样性在堆肥过程中得到恢复。
