Zabarovskiĭ E R
Mol Biol (Mosk). 1986 May-Jun;20(3):639-45.
The possibility of gene suppression by the expression of anti-sense sequences has been tested for tet gene of pBR322 plasmid. Anti-tet gene has been inserted into lac-promoter regulated site of M13mp 10 single-stranded high copy phage vector. To achieve that, HihdIII-BamHI fragment of pBR322 carrying part of the tet gene was inserted into poly-linker of mp 10. The influence of the anti-tet gene expression on growth parameters of cells with or without tetracycline in the growth media was monitored for JM103 cells. The results indicate that in this system the detectable suppression of the tet gene by anti-tet expression was not manifested.
针对pBR322质粒的tet基因，已经测试了通过反义序列表达进行基因抑制的可能性。反tet基因已被插入到M13mp 10单链高拷贝噬菌体载体的lac启动子调控位点。为实现这一点，将携带tet基因部分片段的pBR322的HihdIII - BamHI片段插入到mp 10的多克隆位点。针对JM103细胞，监测了反tet基因表达对生长培养基中有无四环素时细胞生长参数的影响。结果表明，在该系统中，反tet表达对tet基因的可检测抑制并未表现出来。
