Kiselev V I, Rechinskiĭ V O, Nanuĭlov Iu A, Chernaia D S, Ershov Iu V
Mol Gen Mikrobiol Virusol. 1985 Feb(2):14-7.
The spontaneous recovery of activity of tet gene deleted of the promoter region was studied. Plasmid pBRS188 was used as a model for studying this problem. The plasmid has the fragment of tet gene of pBR322, from which it originates, between the sites of restriction endonucleases EcoRI and HindIII cleavage resulting in inactivation of tet promoter. E. coli cells harbouring the plasmid were shown to revert the TcR phenotype with the frequency 10(-9). The gene activation coincided with intraplasmid recombination revealed by restriction analysis. In some cases the recovery of tet gene activity coincided with the formation of multimeric plasmids.
研究了启动子区域tet基因缺失后的自发活性恢复情况。质粒pBRS188被用作研究该问题的模型。该质粒在限制性内切酶EcoRI和HindIII的切割位点之间有其来源的pBR322的tet基因片段，导致tet启动子失活。携带该质粒的大肠杆菌细胞显示出以10(-9)的频率恢复TcR表型。基因激活与通过限制性分析揭示的质粒内重组相一致。在某些情况下，tet基因活性的恢复与多聚体质粒的形成相一致。
