Center of Experimental and Applied Cutaneous Physiology, Department of Dermatology, Charité - Universitätsmedizin Berlin, Germany; Zebet at Federal Institute of Risk Assessment (BfR), Berlin, Germany.
Center of Experimental and Applied Cutaneous Physiology, Department of Dermatology, Charité - Universitätsmedizin Berlin, Germany.
Eur J Pharm Biopharm. 2014 Feb;86(2):301-6. doi: 10.1016/j.ejpb.2013.11.004. Epub 2013 Nov 16.
Liposomes are frequently described as drug delivery systems for dermal and transdermal applications. Recently, it has been shown that particulate substances penetrate effectively into hair follicles and that the follicular penetration depth can be increased by massaging the skin, which simulates the in vivo movement of hairs in the hair follicles. In the present study, massage was applied to skin mounted to Franz diffusion cells. By means of confocal laser scanning microscopy, the influence of massage and occlusion on the follicular penetration depths of rigid and flexible liposomes loaded with a hydrophilic and lipophilic dye was investigated. The application of massage increased follicular penetration significantly. Occlusion resulted in an increased follicular penetration depth only for rigid liposomes, whereas invasomes did not penetrate more effectively if occlusion was applied. The results confirm that massage is an important tool for increasing follicular penetration in ex vivo studies using Franz diffusion cells. Occlusion may reduce the efficacy of follicular penetration depending on the specific liposomal preparation. Rigidity in particular appears to be a relevant parameter.
脂质体常被描述为用于皮肤和经皮应用的药物传递系统。最近，已经表明，颗粒物质可以有效地穿透毛囊，并且通过按摩皮肤可以增加毛囊穿透深度，这模拟了毛发在毛囊中的体内运动。在本研究中，按摩应用于安装在 Franz 扩散细胞上的皮肤。通过共聚焦激光扫描显微镜，研究了按摩和封闭对装载亲水性和亲脂性染料的刚性和柔性脂质体的毛囊穿透深度的影响。按摩的应用显著增加了毛囊的穿透深度。封闭仅对刚性脂质体导致毛囊穿透深度增加，而如果应用封闭，则侵入体并不能更有效地穿透。结果证实，按摩是使用 Franz 扩散细胞进行体外研究中增加毛囊穿透的重要工具。封闭可能会降低特定脂质体制剂的毛囊穿透效果。刚性特别是一个相关的参数。
