Department of Chemistry, The Hong Kong University of Science and Technology, Hong Kong, China.
Eur J Pharm Biopharm. 2011 Sep;79(1):23-7. doi: 10.1016/j.ejpb.2011.03.021. Epub 2011 Mar 30.
Recently, it was demonstrated that particles could be utilized as carrier systems for drugs into the hair follicles. In the present study, a two-component drug delivery system is presented consisting of degradable particles loaded with fluorescein isothiocyanate and a separate protease formulation for degradation. The particles were applied alone, 30 min previous to the protease application and simultaneously with the protease onto porcine skin. Subsequently, biopsies were removed, and the penetration depths of the particles were analyzed using laser scanning microscopy. The obtained results demonstrate that the particles alone achieved a penetration depth of around 900 μm. Similar results were obtained for the successive application of particles and protease, whereas a release of the fluorescent dye was only observed in the upper 250 μm corresponding to the penetration depth of the protease. In the case of the simultaneous application, the particles were partly dissolved before application, leading to a reduced particle size and diminished penetration depth. The results revealed that degradable particles are a promising tool for drug delivery into the skin.
最近，研究表明可以将粒子用作药物进入毛囊的载体系统。在本研究中，提出了一种由负载荧光素异硫氰酸酯的可降解颗粒和用于降解的单独蛋白酶制剂组成的两组件药物递送系统。将粒子单独应用，在蛋白酶应用前 30 分钟和与蛋白酶同时应用于猪皮上。随后，去除活检，并使用激光扫描显微镜分析粒子的穿透深度。获得的结果表明，单独的粒子实现了约 900μm 的穿透深度。连续应用粒子和蛋白酶也获得了类似的结果，而只有在蛋白酶的穿透深度（约 250μm）对应的上层观察到荧光染料的释放。在同时应用的情况下，在应用之前部分溶解了粒子，导致粒子尺寸减小，穿透深度减小。结果表明，可降解颗粒是将药物递送至皮肤的有前途的工具。
