Davoust Bernard, Parzy Daniel, Demoncheaux Jean-Paul, Tine Raphaël, Diarra Mamadou, Marié Jean-Lou, Mediannikov Oleg
Research Unit of Emerging Infectious and Tropical Diseases (URMITE), UMR CNRS 7278 IRD 198, Aix-Marseille-Université, Marseille, France; Animal Epidemiology Working Group of the Military Health Service, Toulon, France.
UMR - MD3, Faculté de pharmacie, Aix-Marseille-Université, Marseille, France; Institut de recherche biomédicale des armées, Brétigny, France.
Comp Immunol Microbiol Infect Dis. 2014 Jan;37(1):31-7. doi: 10.1016/j.cimid.2013.10.005. Epub 2013 Nov 4.
A rapid immuno-migration test for the serological detection of canine monocytic ehrlichiosis, Witness® Ehrlichia (WE) (Zoetis, France), was evaluated in 528 serum samples from dogs living in endemic areas of West and East Africa: Senegal (N=208), Ivory Coast (N=7), Sudan (N=27), and Djibouti (N=286). Of these dogs, 200 were French military working dogs (MWD) temporarily residing in Africa. The WE test results were compared with those obtained by indirect immunofluorescence (IFA). The sensitivity of WE was 97% [94.2, 98.7] with a specificity of 100% [98.6, 100]. In MWD, the seroprevalence (IFA) was 7%; in native dogs, it reached 77.1%. This significant difference can be explained by prophylactic measures from which MWD benefit. The WE test represents a simple, fast and reliable test for the detection of anti-Ehrlichia canis antibodies. Its implementation for the diagnosis of clinical cases has been validated in the field, and its use allows easy detection of asymptomatic dogs that may be carriers of E. canis.
对一种用于犬单核细胞埃立克体病血清学检测的快速免疫迁移试验——Witness®埃立克体（WE）（法国硕腾公司），在来自西非和东非流行地区的528份犬血清样本中进行了评估，这些地区包括塞内加尔（N = 208）、科特迪瓦（N = 7）、苏丹（N = 27）和吉布提（N = 286）。这些犬中，有200只是暂时居住在非洲的法国军犬（MWD）。将WE试验结果与间接免疫荧光法（IFA）获得的结果进行了比较。WE的敏感性为97%[94.2, 98.7]，特异性为100%[98.6, 100]。在军犬中，血清阳性率（IFA）为7%；在本地犬中，血清阳性率达到77.1%。这种显著差异可以通过军犬受益的预防措施来解释。WE试验是一种用于检测犬埃立克体抗体的简单、快速且可靠的试验。其在临床病例诊断中的应用已在现场得到验证，并且使用该试验能够轻松检测出可能是犬埃立克体携带者的无症状犬。
