Laboratory of Genetics and Plant Breeding, College of Agriculture, University of Osaka Prefecture, Sakai, 591, Osaka, Japan.
Theor Appl Genet. 1985 Jun;70(3):271-8. doi: 10.1007/BF00304911.
All of the 21 possible monosomic lines have been screened and confirmed from 33 monosomic stocks of Avena byzantina C. Koch cv. 'Kanota'. All of them, except Mono-21 which was a progeny of monosomic 'Cherokee' (A. sativa) repeatedly backcrossed with 'Kanota', were obtained in the progenies of haploid (2n = 3x), aneuploid (2n = 6X±) and autotriploid (2n = 9X) partners of twins. Identification of the monosomics was carried out by means of the double monosomic method, monosomic analysis on marker genes, leaf peroxidase isozyme analysis, karyotype analysis and nullisomic analysis. The monosomic lines were numbered from Mono-1 through to Mono-21, mainly in the order of monosome length from the longest to the shortest. Most monosomic lines were hardly distinguishable by morphological characteristics from each other or from normal disomics. In the selfed progenies of four monosomic lines, Mono-8, -9, -17 and-19, segregation of nulli-, mono- and disomics was observed, but no nullisomics were found in the other lines. In most cases the frequency of monosomics ranging from 35.5 to 97.8% was, compared to those of nulli- and disomics, highest in the selfed progeny of monosomics. The monosomic lines were easily maintained and can be used for genetic analysis because of their good seed fertility and high monosome transmission rate. They have the near isogenic background of 'Kanota'.
所有 21 条可能的单体系都已通过对 33 条单体资源 'Kanota' 的筛选和鉴定而得到确认，除了 Mono-21，它是单体 'Cherokee'（A. sativa）与 'Kanota' 回交的后代，其余单体都是通过双单体法、单体分析标记基因、叶过氧化物酶同工酶分析、核型分析和单体分析鉴定得到的。单体的编号是从 Mono-1 到 Mono-21，主要是根据单体的长度从最长到最短的顺序。大多数单体系在形态特征上彼此之间或与正常二倍体之间几乎没有区别。在四个单体系（Mono-8、-9、-17 和-19）的自交后代中观察到了单体、缺体和二体的分离，但在其他系中没有发现缺体。在大多数情况下，单体的频率（35.5%至 97.8%）与缺体和二体相比，在单体的自交后代中最高。单体系因其良好的种子育性和高单体传递率而易于维持，并可用于遗传分析。它们具有 'Kanota' 的近等基因背景。
