aEmbrapa Genetic Resources and Biotechnology bInstitute of Biology cCeilandia Faculty, University of Brasilia, Brasilia, DF, Brazil.
Anticancer Drugs. 2014 Mar;25(3):323-31. doi: 10.1097/CAD.0000000000000052.
The present study aimed to entrap and characterize the morphology and antitumor effects of a dermaseptin (DStomo01) peptide in chitosan nanoparticles, in vitro. DStomo01 nanoparticles showed moderate polydispersivity, excellent colloidal stability, and slow release. It was noted that free DStomo01 induced DNA fragmentation and mitochondrial hyperpolarization in HeLa cells. However, when entrapped in chitosan nanoparticles, DStomo01 was slightly more active against HeLa cells than the free peptide. In conclusion, the present sustained release system was efficient in entrapping the peptide and reducing tumor cell viability, which are promising steps for future studies involving specific targeting of nanoparticles and in-vivo treatments.
本研究旨在体外捕获并表征一种抗菌肽（DStomo01）在壳聚糖纳米粒子中的形态和抗肿瘤作用。DStomo01 纳米粒子表现出中等的多分散性、优异的胶体稳定性和缓慢的释放。值得注意的是，游离的 DStomo01 诱导 HeLa 细胞的 DNA 片段化和线粒体超极化。然而，当被包封在壳聚糖纳米粒子中时，DStomo01 对 HeLa 细胞的活性比游离肽略高。总之，本持续释放系统能够有效地捕获肽并降低肿瘤细胞活力，这为未来涉及纳米粒子特异性靶向和体内治疗的研究提供了有希望的步骤。
