Instituto de Química, UNESP, Universidade Estadual Paulista, Rua Prof. Francisco Degni, 55, Araraquara, São Paulo, 14800-060, Brazil.
Bioprocess and Biotechnology Department, FCF, UNESP, Universidade Estadual Paulista, Araraquara, São Paulo, Brazil.
Amino Acids. 2018 May;50(5):503-511. doi: 10.1007/s00726-017-2534-y. Epub 2018 Jan 5.
The biomembrane natural (NRL-Natural Rubber Latex), manipulated from the latex obtained from the rubber tree Hevea brasiliensis, has shown great potential for application in biomedicine and biomaterials. Reflecting the biocompatibility and low bounce rate of this material, NRL has been used as a physical barrier to infectious agents and for the controlled release of drugs and extracts. The aim of the present study was to evaluate the incorporation and release of peptides using a latex biomembrane carrier. After incorporation, the release of material from the membrane was observed using spectrophotometry. Analyses using HPLC and mass spectroscopy did not confirm the release of the antimicrobial peptide [W]Hylin a1 after 24 h. In addition, analysis of the release solution showed new compounds, indicating the degradation of the peptide by enzymes contained in the latex. Additionally, the release of a peptide with a shorter sequence (Ac-WAAAA) was evaluated, and degradation was not observed. These results showed that the use of NRL as solid matrices as delivery systems of peptide are sequence dependent and could to be evaluated for each sequence.
天然生物膜（NRL-天然胶乳）是从橡胶树 Hevea brasiliensis 中提取的胶乳加工而成，具有在生物医药和生物材料中应用的巨大潜力。NRL 体现了这种材料的生物相容性和低反弹率，已被用作阻挡感染性物质和控制药物和提取物释放的物理屏障。本研究旨在评估使用乳胶生物膜载体包载和释放肽类物质。包载后，使用分光光度法观察物质从膜中的释放情况。高效液相色谱法和质谱分析在 24 小时后未确认抗菌肽 [W]Hylin a1 的释放。此外，对释放溶液的分析显示了新的化合物，表明肽被乳胶中含有的酶降解。此外，还评估了具有较短序列（Ac-WAAAA）的肽的释放情况，未观察到降解。这些结果表明，将 NRL 用作肽类物质的固体基质的递送系统是序列依赖性的，并且可以针对每个序列进行评估。
