Department of Horticultural Sciences, New York State Agricultural Experiment Station, Cornell University, 14456, Geneva, NY, USA.
Theor Appl Genet. 1985 Jul;70(4):433-9. doi: 10.1007/BF00273751.
We have attempted to confirm previous reports of "egg transformation" in seven Nicotiana species, including genetic stocks obtained from the original experimenter. The methods employed in the original experiments were duplicated as closely as possible. In total, 1,622 pollinations were made involving irradiated pollen and mixtures of irradiated and self pollen. Consequently, 995 seedlings from 9,052 seeds were screened for 1,594 potentially detectable transformation events. A very low frequency of unexpected progeny resulted, but these results were not repeatable and appear to have arisen by mechanisms other than transformation. These results are strongly at odds with previous claims, when 50% of offspring were found to be transformed. We conclude that the previous observations of high frequency egg transformation are not reproducible. However, due to the ambiguous nature of the markers employed, it is very difficult to prove that such transformation events do not occur as very rare events.
我们曾试图证实先前在 7 种烟草物种中观察到的“卵转化”现象，包括从原始实验者那里获得的遗传品系。我们尽可能地复制了原始实验中使用的方法。总共进行了 1622 次授粉，涉及辐照花粉和辐照花粉与自花粉的混合物。因此，从 9052 颗种子中筛选出了 995 株幼苗，以检测 1594 个潜在的可检测转化事件。结果发现，意想不到的后代出现的频率非常低，但这些结果不可重复，似乎是由转化以外的机制引起的。这些结果与之前 50%的后代发生转化的发现强烈矛盾。我们得出的结论是，之前观察到的高频卵转化现象是不可复制的。然而，由于所使用的标记具有模糊性，很难证明这种转化事件不会以非常罕见的事件发生。
