去盐提取液中分离的保卫细胞原生质体中磷酸烯醇丙酮酸羧化酶的性质。
Properties of phosphoenolpyruvate carboxylase in desalted extracts from isolated guard-cell protoplasts.
机构信息
Institut für Botanik der Technischen Universität, Arcisstrasse 21, D-8000, München 2, Germany.
出版信息
Planta. 1984 Sep;162(3):220-5. doi: 10.1007/BF00397443.
Properties of phosphoenolpyruvate (PEP) carboxylase (EC 4.1.1.31) obtained from isolated guard-cell protoplasts of Vicia faba L. were determined following rapidly desalting of the extract on a Sephadex G 25 column. The activity of PEP carboxylase was measured as a function of PEP and malate concentration, pH and K(+) concentration within 2-3 min after homogenization of the guard-cell protoplasts. The activity of this enzyme was stimulated by PEP concentrations of 0.1 to 0.75 mM and by K(+) ions (12 mM), but inhibited by PEP concentrations above 1 mM and by malate. Changes in the Km(PEP) and Vmax values with increasing malate concentrations (2.5 and 5 mM) indicate that the malate level, varying in relation to the physiological state of guard cells, plays an important role in regulating the properties of phosphoenolpyruvate carboxylase.
从蚕豆保卫细胞原生质体中分离出的磷酸烯醇丙酮酸（PEP）羧化酶（EC 4.1.1.31）的性质，是在 Sephadex G 25 柱上快速脱盐后测定的。PEP 羧化酶的活性是作为 PEP 和苹果酸浓度、pH 值和 K+浓度的函数来测定的，这是在保卫细胞原生质体匀浆后 2-3 分钟内进行的。该酶的活性受到 0.1 至 0.75 mM 的 PEP 浓度和 K+离子（12 mM）的刺激，但受到超过 1 mM 的 PEP 浓度和苹果酸的抑制。随着苹果酸浓度（2.5 和 5 mM）的增加，Km（PEP）和 Vmax 值的变化表明，苹果酸的水平，与保卫细胞的生理状态有关，在调节磷酸烯醇丙酮酸羧化酶的性质方面起着重要作用。
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