Wutzler P, Färber I, Sauerbrei A, Helbig B, Wutke K, Rüdiger K D, Scheibner K, Brichàcek B, Vonka V
Oncology. 1986;43(4):224-9. doi: 10.1159/000226371.
Lymph nodes or tumor biopsies of 60 persons suspected of having a malignant lymphoma were examined for the presence of Epstein-Barr virus nuclear antigen (EBNA) by anticomplement immunofluorescence. In 8 cases the tissue specimens were also assayed for Epstein-Barr virus (EBV) DNA by nucleic acid hybridization. Serum samples of patients and controls were tested for EBV-related antibodies. The histological tests in 37 cases showed a malignant non-Hodgkin lymphoma, and in 23 cases a reactive lymphadenopathy. A Burkitt lymphoma of a European boy and a polymorphic centroblastoma contained EBNA and approximately 27 or 30 genome equivalents EBV DNA per cell, respectively. EBNA was also demonstrated in about 20% of the cells of a lymph node from a patient with recurrent reactive lymphadenopathy.
采用抗补体免疫荧光法，对60名疑似患有恶性淋巴瘤患者的淋巴结或肿瘤活检组织进行检测，以确定是否存在EB病毒核抗原（EBNA）。其中8例组织标本还通过核酸杂交法检测了EB病毒（EBV）DNA。检测了患者和对照者的血清样本中的EBV相关抗体。组织学检查显示，37例为恶性非霍奇金淋巴瘤，23例为反应性淋巴结病。一名欧洲男孩的伯基特淋巴瘤和一例多形性中心母细胞瘤分别含有EBNA，且每个细胞分别含有约27或30个基因组当量的EBV DNA。一名复发性反应性淋巴结病患者的淋巴结中约20%的细胞也检测到了EBNA。
