Olweny C L, Atine I, Kaddu-Mukasa A, Owor R, Andersson-Anvret M, Klein G, Henle W, de-Thé G
J Natl Cancer Inst. 1977 May;58(5):1191-6. doi: 10.1093/jnci/58.5.1191.
Burkitt's lymphoma (BL) has been widely investigated and has attracted attention because of the possible etiologic role of the Epstein-Barr virus (EBV). To further determine the role of EBV in the causation of this tumor, we measured EBV-specific nuclear antigen (EBNA) and EBV DNA using immunofluorescence and nucleic acid hybridization techniques, respectively. Of 34 BL biopsies, 27 tissues (79%) were EBNA-positive, whereas none of the 25 non-BL biopsy tissues were EBNA-positive. Of 15 BL tumors tested, 14 (93%) were EBV DNA-positive with a mean of 39 (range, 8-86) EBV genome equivalents per cell. Each of the 15 non-BL biopsy specimens subjected to nucleic acid hybridization had less than two virus genome equivalents per cell, although all had serologic evidence of past EBV infection. The findings further supported the possible etiologic role of EBV in African BL and negated the passenger hypothesis. The EBV genome could, therefore, be used as a separating marker between African BL and non-BL lymphomas.
伯基特淋巴瘤（BL）已得到广泛研究，并且由于爱泼斯坦-巴尔病毒（EBV）可能的病因学作用而备受关注。为了进一步确定EBV在该肿瘤病因中的作用，我们分别使用免疫荧光和核酸杂交技术检测了EBV特异性核抗原（EBNA）和EBV DNA。在34例BL活检组织中，27例组织（79%）为EBNA阳性，而25例非BL活检组织均为EBNA阴性。在检测的15例BL肿瘤中，14例（93%）为EBV DNA阳性，平均每个细胞有39个（范围为8 - 86个）EBV基因组当量。尽管所有15例接受核酸杂交的非BL活检标本均有既往EBV感染的血清学证据，但每个细胞的病毒基因组当量均少于2个。这些发现进一步支持了EBV在非洲BL中可能的病因学作用，并否定了过客假说。因此，EBV基因组可作为非洲BL与非BL淋巴瘤之间的鉴别标志物。
