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豆细胞质体有被小泡对内化的阳离子化铁蛋白的作用。

Endocytosis of cationized ferritin by coated vesicles of soybean protoplasts.

机构信息

Biology Department, University of Saskatchewan, S7N 0W0, Saskatoon, Sask., Canada.

出版信息

Planta. 1984 Dec;162(6):481-6. doi: 10.1007/BF00399912.

DOI:10.1007/BF00399912
PMID:24253263
Abstract

Soybean (Glycine max (L.) Merr.) protoplasts have been surface-labelled with cationized ferritin, and the fate of the label has been followed ultrastructurally. Endocytosis of the label occurs via the coated-membrane system. The pathway followed by the label, once it has been taken into the interior of the protoplast, appears to be similar to that found during receptor-mediated endocytosis in animal cells. Cationized ferritin is first seen in coated vesicles but rapidly appears in smooth vesicles. Labelled, partially coated vesicles are occasionally observed, indicating that the smooth vesicles may have arisen by the uncoating of coated vesicles. Structures which eventually become labelled with cationized ferritin include multivesicular bodies, dictyosomes, large smooth vesicles, and a system of partially coated reticula.

摘要

大豆(Glycine max(L.)Merr.)原生质体已用阳离子化铁蛋白进行表面标记,并通过超微结构观察了标记的去向。通过有被膜系统内吞标记物。一旦进入原生质体内部,标记物所遵循的途径与动物细胞中受体介导的内吞作用中发现的途径相似。阳离子化铁蛋白首先出现在有被小泡中,但很快出现在光滑小泡中。偶尔观察到标记的部分有被小泡,表明光滑小泡可能是由有被小泡脱被而来。最终用阳离子化铁蛋白标记的结构包括多泡体、高尔基复合体、大的光滑小泡和部分有被网系统。

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The use of lead citrate at high pH as an electron-opaque stain in electron microscopy.在电子显微镜检查中,将高pH值的柠檬酸铅用作电子不透明染色剂。
Protoplasma. 2017 Jan;254(1):75-94. doi: 10.1007/s00709-016-0952-4. Epub 2016 Mar 18.
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Cell wall accumulation of fluorescent proteins derived from a trans-Golgi cisternal membrane marker and paramural bodies in interdigitated Arabidopsis leaf epidermal cells.源自反式高尔基体扁平囊膜标记物的荧光蛋白和互指状拟南芥叶表皮细胞中壁旁体的细胞壁积累。
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