Fukunaga Y, Nagata T, Takebe I, Kakehi T, Matsui C
Exp Cell Res. 1983 Mar;144(1):181-9. doi: 10.1016/0014-4827(83)90452-4.
A one-step procedure using a mixture of glutaraldehyde and osmium tetroxide was devised to fix in situ large unilamellar liposomes of phosphatidylserine for transmission electron microscopy (TEM), since the conventional fixation method was found to be inadequate in this respect. The new fixation procedure enabled us to visualize the sequence of events in the interaction of liposomes with protoplasts from Vinca rosea suspension cultures in the presence of polyethylene glycol. Liposomes were thus found adhering to the surface of protoplasts, in association with invaginating plasmalemma, and within intracellular vesicles. These observations showed that liposomes enter plant protoplasts via endocytosis. Ultrastructural profiles indicating fusion of liposomes with protoplasts were not observed.
由于发现传统固定方法在这方面存在不足，因此设计了一种使用戊二醛和四氧化锇混合物的一步法，用于原位固定磷脂酰丝氨酸的大单层脂质体，以便进行透射电子显微镜（TEM）观察。新的固定方法使我们能够观察到在聚乙二醇存在下脂质体与长春花悬浮培养物原生质体相互作用过程中的一系列事件。结果发现脂质体附着在原生质体表面，与内陷的质膜相关联，并存在于细胞内小泡中。这些观察结果表明脂质体通过内吞作用进入植物原生质体。未观察到表明脂质体与原生质体融合的超微结构特征。
