Department of Soil and Crop Sciences, Texas A&M University, 77843, College Station, Texas, USA.
Plant Cell Rep. 1984 Feb;3(1):41-3. doi: 10.1007/BF00270228.
Stem and petiole sections from a diploid species of cotton (Gossypium klotzschianum Anderss.) were longitudinally sliced and placed on a callus induction medium. Explants with the cut surface in contact with the medium either browned or produced a slow growing red callus which could not be subcultured. Explants oriented with the epidermis in contact with the medium produced a rapidly growing, friable, green callus which could be subcultured biweekly and has been maintained for 12 months. Globular and torpedo embryos were obtained 2 weeks after transfer of the callus to a liquid medium containing the auxin, 2,4-dichlorophenoxyacetic acid. Following subculture to an auxin-free medium, the embryos underwent additional development but plantlets were not obtained. Attempts to consistently regenerate plants from embryos are currently under way.
将二倍体棉花（Gossypium klotzschianum Anderss.）的茎和叶柄部分纵向切片，然后放置在愈伤组织诱导培养基上。与培养基接触的切面要么变褐色，要么产生缓慢生长的红色愈伤组织，无法进行继代培养。与培养基接触的表皮定向的外植体产生快速生长、脆弱、绿色的愈伤组织，可每两周进行一次继代培养，并已维持 12 个月。将愈伤组织转移到含有生长素 2,4-二氯苯氧乙酸的液体培养基中 2 周后，可获得球形和鱼雷形的胚。继代到不含生长素的培养基中后，胚经过进一步发育，但未能获得植株。目前正在尝试从胚中稳定再生植株。
