Laboratorium voor Plantenbiochemie, KULeuven, Kardinaal Mercierlann, 92, B-3030, Leuven, Belgium.
Plant Cell Rep. 1984 Apr;3(2):55-9. doi: 10.1007/BF00270971.
Immature rice (Oryza sativa, L) embryos isolated about 12 days post anthesis are fully able to develop into young seedlings when cultured in vitro. Concomitantly, they rapidly loose their lectin synthesis activity. Abscisic acid added to the nutrient medium prevents precocious germination of the immature embryos and simultaneously strongly promotes lectin biosynthesis activity. Similarly, abscisic acid keeps mature embryos grown in a nutrient medium in a dormant state and maintains their lectin synthesis activity, whereas control embryos rapidly germinate but also quickly loose their lectin synthesis activity. It appears, therefore, that rice lectin is typically synthesized in embryos which are kept in a dornant state.
离体培养的授粉后约 12 天的未成熟水稻(Oryza sativa,L)胚完全能够发育成幼苗。同时,它们迅速失去凝集素合成活性。添加到营养培养基中的脱落酸可防止未成熟胚胎过早发芽,并同时强烈促进凝集素生物合成活性。同样,脱落酸使在营养培养基中生长的成熟胚胎处于休眠状态并保持其凝集素合成活性,而对照胚胎迅速发芽但也迅速失去其凝集素合成活性。因此,似乎水稻凝集素通常在处于休眠状态的胚胎中合成。