El Rabey Haddad A, Al-Sieni Abdulbasit I, Majami Abdullah A
Biochemistry Department, Faculty of Science, King Abdulaziz University, Jeddah, Saudi Arabia Bioinformatics Department, Genetic Engineering and Biotechnology Research Institute, Minufiya University, Sadat City, Egypt
Biochemistry Department, Faculty of Science, King Abdulaziz University, Jeddah, Saudi Arabia.
Toxicol Ind Health. 2014 Nov;30(10):950-63. doi: 10.1177/0748233713505127. Epub 2013 Nov 19.
Screening of the toxic effect of a high oral melamine dose (30,000 ppm supplemented in the diet) was performed for 28 days on male rats. The morphology, anatomy, complete blood count (CBC), serum electrolytes, kidney function, serum proteins, serum bilirubin, serum liver enzymes, catalase, glutathion-S-transferase, lipid peroxide, serum melamine concentration, total body weight, food intake, food efficiency ratio (FER), body weight gain percentage (BWG%), body weight gain, water consumption, and histopathological examinations of kidney, urinary bladder, testis, liver, heart, and spleen were investigated. The melamine-supplemented rats turned yellow and showed different degrees of hypertrophy and congestion, particularly the kidney and the ureter as a result of melamine toxicity. The CBC showed minimal changes in the melamine-supplemented groups. Na and Cl were decreased, whereas K, P, and Ca were increased. Serum creatinine, uric acid, and urea were elevated. Liver function enzymes were nonsignificantly affected. Catalase and glutathion-S-transferase were decreased, whereas lipid peroxide was increased in the kidney tissue homogenate. It was also noted that serum protein was decreased and serum bilirubin was increased. Histopathologically, most examined organs were severely injured specially the kidneys, liver, and testes.
对雄性大鼠进行了为期28天的高剂量口服三聚氰胺（饮食中添加30,000 ppm）毒性作用筛查。研究了其形态学、解剖学、全血细胞计数（CBC）、血清电解质、肾功能、血清蛋白、血清胆红素、血清肝酶、过氧化氢酶、谷胱甘肽-S-转移酶、脂质过氧化物、血清三聚氰胺浓度、总体重、食物摄入量、食物效率比（FER）、体重增加百分比（BWG%）、体重增加、饮水量，以及肾脏、膀胱、睾丸、肝脏、心脏和脾脏的组织病理学检查。补充三聚氰胺的大鼠皮肤变黄，并出现不同程度的肥大和充血，尤其是肾脏和输尿管，这是三聚氰胺毒性作用的结果。CBC显示补充三聚氰胺的组变化极小。钠和氯减少，而钾、磷和钙增加。血清肌酐、尿酸和尿素升高。肝功能酶受到的影响不显著。肾脏组织匀浆中过氧化氢酶和谷胱甘肽-S-转移酶减少，而脂质过氧化物增加。还注意到血清蛋白减少，血清胆红素增加。组织病理学检查显示，大多数检查的器官受到严重损伤，尤其是肾脏、肝脏和睾丸。
