Institut für Botanik der universität Hannover, Herrenhäuser Straße 2, D-3000, Hannover, Federal Republic of Germany.
Plant Cell Rep. 1984 Jun;3(3):81-4. doi: 10.1007/BF02441004.
The isolation of polynucleotide phosphorylase (EC 2. 7. 7. 8) from suspension cultured plant cells of parsley (Petroselinum sativum) and from tomato seedlings (Lycopersicon esculentum) is described. The procedure includes an ultracentrifugation step, a glycerol density gradient centrifugation and preparative gel electrophoresis under nondenaturing conditions. Isoelectric focusing gives rise to a major component (pI ≈ 7.5) and to a minor one (pI ≈ 5). The enzyme contains five subunits with apparent Mr values of 160 000, 140 000, 70 000, 34 000 and 12 000, the 70 000-dalton one being a glycoprotein.
从欧芹（Petroselinum sativum）悬浮培养植物细胞和番茄幼苗（Lycopersicon esculentum）中分离多核苷酸磷酸化酶（EC 2. 7. 7. 8）的方法。该方法包括超速离心步骤、甘油密度梯度离心和非变性条件下的制备性凝胶电泳。等电聚焦产生主要成分（pI≈7.5）和次要成分（pI≈5）。该酶包含五个亚基，表观 Mr 值分别为 160000、140000、70000、34000 和 12000，70000 道尔顿的亚基是糖蛋白。
