急性心肌梗死和稳定型冠心病中的循环微RNA（miR-423-5p、miR-208a和miR-1）

Circulating microRNAs (miR-423-5p, miR-208a and miR-1) in acute myocardial infarction and stable coronary heart disease.

作者信息

Nabiałek E, Wańha W, Kula D, Jadczyk T, Krajewska M, Kowalówka A, Dworowy S, Hrycek E, Włudarczyk W, Parma Z, Michalewska-Włudarczyk A, Pawłowski T, Ochała B, Jarząb B, Tendera M, Wojakowski W

机构信息

Third Division of Cardiology Medical University of Silesia, Katowice, Poland -

出版信息

Minerva Cardioangiol. 2013 Dec;61(6):627-37.

PMID:24253456

Abstract

AIM

The microRNAs (miRs) are small non-coding RNAs which regulate expression of multiple genes involved in atherogenesis. MicroRNA are also present in circulation. The aims of this study were: 1) assessment of expression level of miR-1, miR-208a and miR-423-5p in plasma in patients with STEMI, stable CAD and healthy individuals; 2) evaluation of correlation between plasma miRs and left ventricle ejection fraction, end- systolic and end-diastolic diameters and troponin release in patients with STEMI.

METHODS

Study group consisted of 26 patients: 1) acute MI group (N.=17); 2) stable CAD group (N.=4); and 3) subjects with no history of CAD (control group, N.=5). Expression of miR-423-5p, miR-208 and miR-1 was measured in plasma before PCI, 6, 12 and 24 hours later. Expression level ofmiRs was measured using TaqMan® MicroRNA Assays. Expression was assessed by Pfaffl method, and miR-39 was used for normalization of the results.

RESULTS

In stable CAD in comparison to control group the expression level of miR-1, miR-208a and miR-423-5p did not show significant differences. Also there was no significant increase of number of miR copies at 6, 12 and 24 hours after PCI. There was a significantly higher number of miR-423-5p copies in patients with acute MI before the pPCI. After 6, 12 and 24 hours post-procedure the expression level was similar to the control group and significantly lower than the baseline level. Conversely, the expression level of miR-1 and miR-208a were not significantly different than in the control group. In patients with acute MI there were no significant correlations between the expression level of miRs and any of the echocardiographic parameters of LV as well as level of troponin I at any time-point of the follow-up.

CONCLUSION

Early in acute myocardial infarction the expression of miR-423-5p in plasma is significantly increased with subsequent normalization within 6 hours. Potentially it is an early marker of myocardial necrosis.

摘要

目的

微小RNA（miRs）是一类小的非编码RNA，可调节参与动脉粥样硬化形成的多个基因的表达。微小RNA也存在于循环系统中。本研究的目的是：1）评估ST段抬高型心肌梗死（STEMI）患者、稳定型冠心病患者及健康个体血浆中miR-1、miR-208a和miR-423-5p的表达水平；2）评估STEMI患者血浆miRs与左心室射血分数、收缩末期和舒张末期直径以及肌钙蛋白释放之间的相关性。

方法

研究组包括26例患者：1）急性心肌梗死组（n = 17）；2）稳定型冠心病组（n = 4）；3）无冠心病病史的受试者（对照组，n = 5）。在PCI术前、术后6小时、12小时和24小时测量血浆中miR-423-5p、miR-208和miR-1的表达。使用TaqMan®微小RNA检测法测量miRs的表达水平。采用Pfaffl法评估表达，并使用miR-39对结果进行标准化。

结果

与对照组相比，稳定型冠心病患者中miR-1、miR-208a和miR-423-5p的表达水平无显著差异。PCI术后6小时、12小时和24小时miR拷贝数也无显著增加。急性心肌梗死患者在pPCI术前miR-423-5p拷贝数显著更高。术后6小时、12小时和24小时，其表达水平与对照组相似，且显著低于基线水平。相反，miR-1和miR-208a的表达水平与对照组无显著差异。在急性心肌梗死患者中，miRs的表达水平与随访任何时间点左心室的任何超声心动图参数以及肌钙蛋白I水平均无显著相关性。