Shanghai Institute of Plant Physiology, Academia Sinica, Shanghai, China.
Plant Cell Rep. 1984 Aug;3(4):149-50. doi: 10.1007/BF00270210.
Young inflorescence explants of Setaria italica in culture showed high capacity for regenerating plantlets through somatic embryogenesis. Embryogenic callus formation was initiated from the explants cultured on Murashige and Skoog's medium with 2 mg/l 2,4-D and 0.2-0.5 mg/l KT or BAP, but it was better for the maintenance of embryogenic growth to subculture the calli on the medium with 2,4-D and KT/BAP and on the medium with 2 mg/l 2iPA and 0.2 mg/l NAA alternately. A number of plantlets were regenerated when embryogenic calli were transferred onto the same basic medium but with 2 mg/l BAP and 0.5 mg/l NAA. Plant regeneration capacity has been maintained in some embryogenic calli during fourteen months of subculture.
年轻的狗尾草花序外植体在培养中表现出通过体细胞胚胎发生再生植物的高能力。胚性愈伤组织的形成是从在含有 2 mg/L 2,4-D 和 0.2-0.5 mg/L KT 或 BAP 的 Murashige 和 Skoog 培养基上培养的外植体开始的，但更好的是将愈伤组织在含有 2,4-D 和 KT/BAP 的培养基和含有 2 mg/L 2iPA 和 0.2 mg/L NAA 的培养基上交替培养，以维持胚性生长。当胚性愈伤组织转移到含有 2 mg/L BAP 和 0.5 mg/L NAA 的相同基本培养基上时，会再生出许多植物。在 14 个月的继代培养中，一些胚性愈伤组织保持了植物再生能力。
