Institute of Botany, Academia Sinica, Taipei, Taiwan, Republic of China.
Theor Appl Genet. 1986 Dec;73(2):161-3. doi: 10.1007/BF00289269.
Young inflorescence explants of green bamboo (Bambusa oldhamii Munro) in culture show a high capacity for plant regeneration through somatic embryogenesis. Embryogenic callus was initiated from explants maintained on Murashige and Skoog's medium supplemented with 3 mg/l 2,4-D, 2 mg/l kinetin and a high content (60 g/l) of sucrose. Prolonged culture in the embryoid induction medium or transferral of embryonic callus to auxin-free medium resulted in the continued development and eventual germination of embryoids and establishment of rooted plantlets that were successfully transferred to soil.
幼嫩的绿竹(Bambusa oldhamii Munro)花序外植体在培养中通过体细胞胚胎发生显示出很高的植物再生能力。胚性愈伤组织是从在添加 3 mg/l 2,4-D、2 mg/l 激动素和高浓度(60 g/l)蔗糖的 Murashige 和 Skoog 培养基上培养的外植体中诱导产生的。在胚状体诱导培养基中延长培养或将胚胎愈伤组织转移到不含生长素的培养基中,导致胚状体的持续发育和最终萌发,并建立了生根的小植株,这些小植株成功地移栽到土壤中。