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通过绿竹(Bambusa oldhamii Munro)愈伤组织培养中的体细胞胚胎发生进行植株再生。

Plant regeneration through somatic embryogenesis in callus culture of green bamboo (Bambusa oldhamii Munro).

机构信息

Institute of Botany, Academia Sinica, Taipei, Taiwan, Republic of China.

出版信息

Theor Appl Genet. 1986 Dec;73(2):161-3. doi: 10.1007/BF00289269.

DOI:10.1007/BF00289269
PMID:24240845
Abstract

Young inflorescence explants of green bamboo (Bambusa oldhamii Munro) in culture show a high capacity for plant regeneration through somatic embryogenesis. Embryogenic callus was initiated from explants maintained on Murashige and Skoog's medium supplemented with 3 mg/l 2,4-D, 2 mg/l kinetin and a high content (60 g/l) of sucrose. Prolonged culture in the embryoid induction medium or transferral of embryonic callus to auxin-free medium resulted in the continued development and eventual germination of embryoids and establishment of rooted plantlets that were successfully transferred to soil.

摘要

幼嫩的绿竹(Bambusa oldhamii Munro)花序外植体在培养中通过体细胞胚胎发生显示出很高的植物再生能力。胚性愈伤组织是从在添加 3 mg/l 2,4-D、2 mg/l 激动素和高浓度(60 g/l)蔗糖的 Murashige 和 Skoog 培养基上培养的外植体中诱导产生的。在胚状体诱导培养基中延长培养或将胚胎愈伤组织转移到不含生长素的培养基中,导致胚状体的持续发育和最终萌发,并建立了生根的小植株,这些小植株成功地移栽到土壤中。

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本文引用的文献

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Plant Cell Rep. 1985 Aug;4(4):191-4. doi: 10.1007/BF00269286.
通过……的茎尖进行愈伤组织诱导和再生。 你提供的原文不完整,请补充完整以便我能更准确地翻译。
Springerplus. 2016 Oct 18;5(1):1799. doi: 10.1186/s40064-016-3520-7. eCollection 2016.
4
Limitations, progress and prospects of application of biotechnological tools in improvement of bamboo-a plant with extraordinary qualities.生物技术工具在改良竹子(一种具有非凡特性的植物)中的应用局限、进展与前景
Physiol Mol Biol Plants. 2013 Jan;19(1):21-41. doi: 10.1007/s12298-012-0147-1.
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In vitro propagation of the bamboo (Bambusa tulda Roxb.) through shoot proliferation.通过芽增殖进行竹子(Bambusa tulda Roxb.)的离体繁殖。
Plant Cell Rep. 1990 Dec;9(8):431-4. doi: 10.1007/BF00232266.
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Plant Cell Rep. 1992 Jun;11(5-6):257-61. doi: 10.1007/BF00235077.
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