Research Center for Cell and Tissue Culture, Kyoto University, Kyoto, Japan.
Plant Cell Rep. 1984 Oct;3(5):186-8. doi: 10.1007/BF00270196.
Calluses were derived from explants of aerial parts of a Duboisia leichhardtii F. Muell. plant. After 3 months of culture we detected small amounts of hyoscyamine and scopolamine in the unorganized calluses, but the amounts decreased until they were undetectable with successive subculture. When shoots were produced from the unorganized calluses, the ability to convert hyoscyamine added into media to scopolamine appeared; when roots were initiated from the shoot-organizing calluses, hyoscyamine, scoporamine, and nicotine were synthesized. Cultured adventitious roots produced these alkaloids and converted exogenous hyoscyamine to scopolamine.
愈伤组织来源于醉茄Duboisia leichhardtii F. Muell. 植物的地上部分外植体。培养 3 个月后，我们在无组织的愈伤组织中检测到少量的莨菪碱和东莨菪碱，但随着继代培养的进行，其含量逐渐减少，直至无法检测到。当不定芽从无组织的愈伤组织中产生时，将添加到培养基中的莨菪碱转化为东莨菪碱的能力出现；当从芽组织愈伤组织中诱导生根时，合成了莨菪碱、东莨菪碱和尼古丁。培养的不定根产生这些生物碱，并将外源性莨菪碱转化为东莨菪碱。
