Griffin W J, Brand H P, Dare J G
J Pharm Sci. 1975 Nov;64(11):1821-5. doi: 10.1002/jps.2600641115.
Duboisia samples were analyzed for scopolamine and hyoscyamine using GLC, by a slope ratio method, and by methods employing homatropine, tetraphenylethylene, and phenylacetyltropine as internal standards. Limits of precision were determined by the inverse prediction method. Phenylacetyltropine was the preferred internal standard. The alkaloids were silanized with either hexamethyldisilazane or N,O-bis(trimethylsily)acetamide to prevent dehydration to the apo forms. Samples from a commercial bale of Duboisia myoporoides were assayed. The alkaloid content varied considerably, depending on the sample position within the bale.
使用气相色谱法、斜率比法以及采用后马托品、四苯乙烯和苯乙酰托品作为内标的方法，对澳洲茄属样本中的东莨菪碱和莨菪碱进行了分析。通过反向预测法确定了精密度限度。苯乙酰托品是首选的内标。用六甲基二硅氮烷或N,O-双（三甲基硅基）乙酰胺对生物碱进行硅烷化处理，以防止脱水形成去水形式。对一捆商业性的澳洲茄样本进行了分析。生物碱含量差异很大，这取决于样本在捆中的位置。
