Advanced Genetic Sciences, Inc., P.O. Box 1373, 66502, Manhattan, KS, USA.
Plant Cell Rep. 1984 Oct;3(5):196-8. doi: 10.1007/BF00270199.
Fusion of freely suspended protoplast mixtures (hypocotyl protoplasts of Brassica napus mixed with mesophyll protoplasts of either B. campestris or Nicotiana plumbaginifolia) was induced by a solution containing 10% polyethylene glycol, 10% dimethyl-sulfoxide and 0.1M glycine-NaOH buffer (pH 10.0). The fusion products represented 15 to 17 percent of the surviving cells. More than 50% of the fusion products divided within two days after fusion, indicating that the fusion procedure did not significantly affect the viability of fused cells. The fusion products were not bound to the surface of the fusion vessel, so they could be isolated with a micropipette immediately after fusion.
自由悬浮原生质体混合物（甘蓝型油菜下胚轴原生质体与白菜或烟草叶片原生质体混合）在含有 10%聚乙二醇、10%二甲基亚砜和 0.1M 甘氨酸-氢氧化钠缓冲液（pH 值 10.0）的溶液中诱导融合。融合产物占存活细胞的 15%至 17%。融合后两天内，超过 50%的融合产物分裂，表明融合过程并未显著影响融合细胞的活力。融合产物不与融合容器表面结合，因此可以在融合后立即用微吸管分离。
