Donahue P R, Palmer D K, Condie J M, Sabatini L M, Blumenfeld M
Proc Natl Acad Sci U S A. 1986 Jul;83(13):4744-8. doi: 10.1073/pnas.83.13.4744.
Drosophila chromatin contains two antigenically distinct H2A histones, H2A.1 and H2A.2. Indirect immunofluorescence analyses revealed that anti-H2A.1 binding was distributed throughout polytene chromosomes, whereas anti-H2A.2 binding was interband-specific. Thus, H2A.2 probably contributes to the less compacted structure of interbands. Since each band-interband region is thought to contain a single gene, our results suggest that the distribution of H2A.2 echoes the functional organization of the Drosophila genome. Similar H2A histones occur in eukaryotes ranging from protozoa to mammals. Their placement might be an important determinant of chromatin structure.
果蝇染色质包含两种抗原性不同的H2A组蛋白，即H2A.1和H2A.2。间接免疫荧光分析显示，抗H2A.1的结合分布于整个多线染色体，而抗H2A.2的结合具有间带特异性。因此，H2A.2可能促成了间带结构较松散的结构。由于每个带 - 间带区域被认为包含单个基因，我们的结果表明H2A.2的分布反映了果蝇基因组的功能组织。从原生动物到哺乳动物的真核生物中都存在类似的H2A组蛋白。它们的定位可能是染色质结构的一个重要决定因素。
Zotero 插件
