Carr A M, Dorrington S M, Hindley J, Phear G A, Aves S J, Nurse P
Imperial Cancer Research Fund, London, UK.
Mol Gen Genet. 1994 Dec 1;245(5):628-35. doi: 10.1007/BF00282226.
We have isolated and characterised the pht1 gene from the fission yeast Schizosaccharomyces pombe. The sequence of the predicted translation product has revealed a striking similarity to the family of H2A.F/Z histone variant proteins, which have been found in a variety of different organisms. Cells deleted for the pht1 gene locus grow slowly, exhibit an altered colony morphology, increased resistance to heat shock and show a significant decrease in the fidelity of segregation of an S. pombe minichromosome. We propose that the histone H2A variant encoded by the pht1 gene is important for chromosomal structure and function, possibly including a role in controlling the fidelity of chromosomal segregation during mitosis.
我们已经从裂殖酵母粟酒裂殖酵母中分离并鉴定了pht1基因。预测翻译产物的序列显示与H2A.F/Z组蛋白变体蛋白家族有显著相似性，这些蛋白已在多种不同生物体中被发现。缺失pht1基因座的细胞生长缓慢，菌落形态改变，对热休克的抗性增加，并且粟酒裂殖酵母微型染色体分离的保真度显著降低。我们提出，由pht1基因编码的组蛋白H2A变体对染色体结构和功能很重要，可能在控制有丝分裂期间染色体分离的保真度方面发挥作用。
