Department of Molecular Biology, University of Geneva, 30, quai Ernest-Ansermet, CH-1211 Geneva 4, Switzerland.
EMBO J. 1984 Nov;3(11):2537-41. doi: 10.1002/j.1460-2075.1984.tb02169.x.
Transcripts from different tissues were mapped along a 315 kb segment of the Drosophila chromosome, a region which includes the rosy and Ace loci. Forty-three distinct RNA species were detected, though only 12 recessive lethal complementation groups had been mapped in the interval. The sum of the sizes of the transcripts covers 33% of the genomic DNA. The distribution of transcription units along the walk is very uneven. Sixty-three kb of genomic DNA at the proximal end of the walk encode 18 transcripts, while only seven are found in the next 153 kb. Each tissue exhibits a specific spectrum of transcripts. No clustering was seen among genes expressed coordinately. In salivary glands, the number of transcripts detected corresponds to the number of chromomeric units in the polytene chromosomes of this tissue. Moreover, the density distribution of transcripts along the DNA walk is parallel to the density distribution of chromomeric units.
来自不同组织的转录本沿着果蝇染色体的 315kb 片段被定位，该区域包括 rosy 和 Ace 基因座。尽管在该区间已经映射了 43 种不同的 RNA 种类，但只有 12 个隐性致死互补群被映射。转录本的大小总和覆盖了基因组 DNA 的 33%。转录单位在步行过程中的分布非常不均匀。步行近端的 63kb 基因组 DNA 编码 18 个转录本，而在接下来的 153kb 中只有 7 个。每个组织都表现出特定的转录本谱。没有观察到协调表达的基因之间的聚类。在唾液腺中，检测到的转录本数量与该组织的多线染色体中的染色粒单元数量相对应。此外，转录本沿 DNA 步行的密度分布与染色粒单元的密度分布平行。
