一种从各种植物来源中分离可限制的线粒体 DNA 的快速廉价方法。

A rapid inexpensive method for the isolation of restrictable mitochondrial DNA from various plant sources.

机构信息

Plant Pathology Department, University of Florida, 32611, Gainesville, Florida, USA.

出版信息

Plant Cell Rep. 1984 Dec;3(6):237-9. doi: 10.1007/BF00269301.

Abstract

A simplified method for the isolation of mitochondrial DNA (mtDNA) of several plant species from either coleoptile or tissue cultured cells is described. The procedure does not require gradient ultracentrifugation or organic solvent extractions (such as phenol, chloroform, ether, etc.). Protoplast isolation is not required for the release of organelles from cell suspension cultured cells. The entire procedure can be performed in a single day and employs differential low speed centrifugations for isolation of mitochondria and differential precipitations for the recovery of restrictable DNA.

摘要

本文描述了一种从 coleoptile 或组织培养细胞中分离几种植物的线粒体 DNA（mtDNA）的简化方法。该方法不需要梯度超速离心或有机溶剂提取（如酚、氯仿、乙醚等）。对于从细胞悬浮培养细胞中释放细胞器，不需要原生质体分离。整个过程可以在一天内完成，并采用差速低速离心分离线粒体和差速沉淀回收限制性 DNA。