Southern Regional Research Center, P.O. Box 19687, 70179, New Orleans, LA, USA.
Planta. 1984 May;161(3):281-7. doi: 10.1007/BF00982926.
The reaction of N,N-diethylaziridinium chloride with raw cotton (Gossypium hirsutum L.) seed fibers to introduce N,N-diethylaminoethyl (DEAE) substituents at a low degree of substitution was used for demonstrating the presence of O(4)H, characteristic of a β-1,3-glucan. The derivatized 1,3-glucan/cellulose was hydrolyzed to DEAE-glucoses that were analyzed by gas-liquid chromatography. Capillary columns proved effective for measuring the small amounts of 4-O-DEAE-glucose in the presence of major amounts of 2-O- and 6-O-DEAE-glucoses. Analyses of raw cotton fibers were carried out through fiber development (20, 27, 34, 41 and 48 d post anthesis, DPA) and field exposure (62, 83 and 104 DPA) periods. The yields of 4-O- and other individual DEAE-glucoses and the yield of 4-O-DEAE-glucose in relation to 2-O-DEAE-glucose were particularly informative concenring the role of the β-1,3-glucan in cellulose. The results confirmed the early production and almost immediate decrease of the β-1,3-glucan and demonstrated continued production of accessible cellulose followed by a sharp decrease in accessibility after boll opening. The β-1,3-glucan content of the raw cotton fiber, estimated from the yield of 4-O-DEAE-glucose (representing 1,3-glucan) and the yield of 2-O-DEAE-glucose (approximating 1,3-glucan plus cellulose) was 10%, 4%, 1% and 0.6% at, in the order given, 20, 27, 48, and 104 DPA. These results are in general agreement with other conventional analyses.
N,N-二乙基氮丙啶氯化物与原棉（棉属植物）种子纤维的反应，以在低取代度下引入 N,N-二乙氨基乙基（DEAE）取代基，用于证明存在 O(4)H，这是β-1,3-葡聚糖的特征。衍生的 1,3-葡聚糖/纤维素被水解为 DEAE-葡萄糖，通过气相色谱进行分析。毛细管柱对于在大量 2-O-和 6-O-DEAE-葡萄糖存在的情况下测量少量 4-O-DEAE-葡萄糖非常有效。通过纤维发育（开花后 20、27、34、41 和 48 天，DPA）和田间暴露（62、83 和 104 DPA）时期对原棉纤维进行分析。4-O-和其他单个 DEAE-葡萄糖的产率以及 4-O-DEAE-葡萄糖与 2-O-DEAE-葡萄糖的产率特别有助于了解β-1,3-葡聚糖在纤维素中的作用。结果证实了β-1,3-葡聚糖的早期产生和几乎立即减少，并证明了在棉铃开放后可及性急剧下降之前，可及纤维素的持续产生。从 4-O-DEAE-葡萄糖（代表 1,3-葡聚糖）和 2-O-DEAE-葡萄糖（近似于 1,3-葡聚糖加纤维素）的产率估算的原棉纤维中的β-1,3-葡聚糖含量，在以下顺序中分别为 10%、4%、1%和 0.6%：20、27、48 和 104 DPA。这些结果与其他常规分析基本一致。
