Plant Pathology Laboratory, Faculty of Agriculture, Nagoya University, 464, Nagoya, Japan.
Plant Cell Rep. 1985 Apr;4(2):74-7. doi: 10.1007/BF00269210.
Within about 10 days after inoculation with Agrobacterium rhizogenes, the vascular bundles of storage root disks of turnip or radish developed small outgrowths with numerous root hairs. Thereafter, adventitious roots (hairy roots) emerged extensively from these outgrowths. The hairy roots which emerged fully supported the growth of host plants, though they lacked geotropism. An excised hairy root could be subcultured as an axenic root culture in the absence of phytohormones. Hairy root cultures with extensive lateral branches grew much more rapidly than those with few lateral branches or ordinary roots. Calli were induced from hairy root cultures in the presence of 2,4-D, and root proliferation from these calli occurred in the absence of 2,4-D. Both the primary hairy roots and the roots which grew from them synthesized agropine and mannopine.
在接种发根农杆菌后大约 10 天，萝卜或大头菜贮藏根盘的维管束发育出带有许多根毛的小突起。此后，这些突起中广泛地长出了不定根（毛状根）。这些完全长出的毛状根支持着宿主植物的生长，尽管它们缺乏向地性。离体的毛状根可以在没有植物激素的情况下作为无菌根培养物进行继代培养。具有广泛侧枝的毛状根培养物比具有少量侧枝或普通根的毛状根培养物生长得更快。在 2,4-D 的存在下，可以从毛状根培养物中诱导出愈伤组织，并且这些愈伤组织在没有 2,4-D 的情况下会发生根的增殖。原初的毛状根和由其生长的根都合成农杆碱和甘露碱。
