原松苗质体中 3-吲哚乙酸的分解代谢。

Catabolism of 3-indole acetic acid in protoplasts from etiolated seedlings of scots pine (Pinus sylvestris L.).

机构信息

Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences, S-901 83, Umeå, Sweden.

出版信息

Plant Cell Rep. 1985 Apr;4(2):100-3. doi: 10.1007/BF00269217.

DOI:10.1007/BF00269217

PMID:24253695

Abstract

Protoplasts preparated from dark grown seedlings of Pinus sylvestris L. were incubated with 3-indole (1-(14)C) acetic acid and 3-indole (2-(14)C) acetic acid (IAA). Three catabolites were consistently produced in the (2-(14)C) IAA feeds, one of which co-chromatographed with 3-indole methanol on reversed phase high performance liquid chromatography (HPLC). Protoplasts feed with (1-(14)C) IAA produced only one labelled catabolite. The non-decarboxylated compound formed was highly polar on reversed phase HPLC, both in the ion suppression and the ion pair mode. The substance was not hydrolysable at pH 11 and 100° indicating that it is not a conjugated form. Effects of time of incubation, pH and the cofactors hydrogen peroxide and 2,4-dichlorphenol on the catabolic rate of IAA are discussed.

摘要

从黑暗中生长的欧洲赤松幼苗中制备的原生质体用 3-吲哚（1-（14）C）乙酸和 3-吲哚（2-（14）C）乙酸（IAA）孵育。在（2-（14）C）IAA 饲料中始终产生三种代谢产物，其中一种与反相高效液相色谱（HPLC）上的 3-吲哚甲醇共色谱。用（1-（14）C）IAA 喂养的原生质体仅产生一种标记的代谢产物。形成的非脱羧化合物在反相 HPLC 中具有很高的极性，无论是在离子抑制还是离子对模式下。该物质在 pH 值为 11 和 100°C 下不可水解，表明它不是一种共轭形式。讨论了孵育时间、pH 值以及过氧化氢和 2,4-二氯苯酚等辅助因子对 IAA 代谢速率的影响。