从杂种松细胞悬浮液中分离和培养原生质体

Isolation and growth of protoplasts from cell suspensions of Pinus contorta dougl. ex loud.

机构信息

Institute of Physiological Botany, University of Uppsala, Box 540, S-75121, Uppsala, Sweden.

出版信息

Plant Cell Rep. 1983 Apr;2(2):92-4. doi: 10.1007/BF00270174.

Abstract

Cell suspensions were initiated from embryo derived calli of Pinus contorta. Some of these cell lines could be maintained in culture for at least one year without reduced growth.A high yield of protoplasts was obtained from the cell suspensions. The protoplasts started to divide after two days and cell clusters could be observed after about two weeks. The growth phase of the cell suspensions was very important for the division of protoplasts. Only protoplasts isolated from suspensions in an actively dividing phase were able to divide with a high frequency and to give rise to cell clusters.

摘要

细胞悬浮液由火炬松胚衍生愈伤组织启动。这些细胞系中的一些可以在培养中至少维持一年而不降低生长速度。从细胞悬浮液中获得了很高的原生质体产量。原生质体在两天后开始分裂，大约两周后可以观察到细胞团。细胞悬浮液的生长阶段对原生质体的分裂非常重要。只有从处于活跃分裂阶段的悬浮液中分离出的原生质体才能以高频率分裂并产生细胞团。