鉴定和量化黑暗中生长的欧洲赤松（Pinus sylvestris L.）幼苗中的吲哚-3-甲醇。
Identification and Quantification of Indole-3-methanol in Etiolated Seedlings of Scots Pine (Pinus sylvestris L.).
机构信息
Department of Forest Genetics and Plant Physiology, The Swedish University of Agricultural Sciences, S-901 83 Umeå, Sweden.
出版信息
Plant Physiol. 1985 Apr;77(4):952-5. doi: 10.1104/pp.77.4.952.
Abstract
Combined gas chromatography-mass spectrometry has been used to identify indole-3-methanol in a purified buffer extract from etiolated seedlings of Pinus sylvestris L. Quantitative estimates obtained by high performance liquid chromatography with fluorescence detection, corrected for losses occurring during purification, indicated that etiolated seedlings of P. sylvestris contained 19.7 +/- 1.4 nanograms (+/- standard deviation) indole-3-methanol per gram fresh weight. The stability of indole-3-methanol at different pH levels was investigated. The rate of conversion, to a less polar unidentified substance, was enhanced with increasing acidity.
摘要
联合气相色谱-质谱法已被用于鉴定从欧洲赤松幼苗的纯化缓冲提取物中的吲哚-3-甲醇。通过高效液相色谱法与荧光检测进行定量估计，并对纯化过程中发生的损失进行了校正，结果表明欧洲赤松幼苗每克鲜重含有 19.7 +/- 1.4 纳克吲哚-3-甲醇。研究了吲哚-3-甲醇在不同 pH 值下的稳定性。随着酸度的增加，向极性较低的未知物质转化的速度加快。
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