Biologisches Institut der Universität II, Schänzlestrasse 1, D-7800, Freiburg, Federal Republic of Germany.
Planta. 1984 Jul;161(5):444-50. doi: 10.1007/BF00394576.
The transcription rate of isolated nuclei from oat seedlings was analysed by measuring the incorporation of radioactive label in trichloroacetic-acid-pelletable material. It was observed that this transcription rate depends on the light pretreatment of the plants: 7.5 h after a 5-min red or far-red light pulse maximum rates were found, but at this time far-red light induced only about half of the stimulation observed after a red light pulse. The far-red reversibility of the effect of the red light pulse indicates that phytochrome controls the capacity for transcription by isolated nuclei. Besides this slow reaction, phytochrome regulated the transcription rates in a very fast response when homogenates were irradiated.
用放射性标记在三氯乙酸可沉淀物质中的掺入来分析从小麦苗中分离出来的细胞核的转录率。观察到，这种转录率取决于植物的光预处理：红光或远红光脉冲 5 分钟后 7.5 小时达到最大速率，但此时远红光诱导的刺激仅约为红光脉冲后观察到的一半。红光脉冲效应的远红光可逆性表明，光敏色素通过分离的核控制转录能力。除了这种缓慢的反应外，当匀浆照射时，光敏色素还通过非常快速的反应来调节转录速率。
