Biologisches Institut II der Universität, Schänzlestrasse 1, D-7800, Freiburg/Br., Germany.
Planta. 1983 Apr;157(5):392-400. doi: 10.1007/BF00397196.
The phytochrome content, the rate of phytochrome accumulation after a light/dark transition and the rate of phytochrome destruction after a 1.5 d reaccumulation period in darkness were measured in light grown Avena sativa L. seedlings. The results using spectrophotometrical methods (Norflurazon treated seedlings) and the radio-immunoassay (RIA) (green seedlings) were almost identical. The rate of phytochrome synthesis was analysed by measuring the activity of poly(A(+))-RNA coding for the phytochrome apoprotein. It was demonstrated that the rate of phytochrome synthesis is different in light and in dark. These results were confirmed by measuring the incorporation of radioactive label in vivo. Five minutes red (and 5 min far-red) light strongly reduces the rate of phytochrome synthesis. Even after prolonged dark periods only 50% of the initial rate of phytochrome synthesis is recovered for light and dark grown seedlings which received one red light pulse.
在光生长的燕麦（Avena sativa L.）幼苗中，测定了光敏色素含量、光/暗转换后光敏色素积累的速率以及黑暗中重新积累 1.5 天（d）后光敏色素的破坏速率。使用分光光度法（诺氟拉宗处理的幼苗）和放射免疫测定法（RIA）（绿色幼苗）得到的结果几乎完全一致。通过测量编码光敏色素脱辅基蛋白的 poly(A(+))-RNA 的活性来分析光敏色素的合成速率。结果表明，在光照和黑暗条件下，光敏色素的合成速率不同。这些结果通过体内放射性标记的掺入得到了证实。5 分钟红光（和 5 分钟远红光）强烈降低了光敏色素的合成速率。即使在长时间的黑暗期后，对于接受过一次红光脉冲的光和暗生长的幼苗，光敏色素的合成速率也仅恢复到初始速率的 50%。
