Laboratoire de Photosynthèse, CNRS, F-91190, Gif-sur-Yvette, France.
Theor Appl Genet. 1985 Jul;69(4):361-6. doi: 10.1007/BF00570903.
The mitochondrial genomes of five rapeseed somatic hybrid plants, which combine in a first experimentBrassica napus chloroplasts and a cytoplasmic male sterility trait coming fromRaphanus sativus, and in a second experiment chloroplasts of a triazine resistantB. compestris and a cytoplasmic male sterility trait fromR. sativus, were analyzed by restriction endonucleases. Restriction fragment patterns indicate that these genomes differ from each other and from both parents. The presence of new bands in the somatic hybrid mitochondrial DNA restriction patterns is evidence of mitochondrial recombination in somatic hybrid cells. In both parental and somatic hybrid plants large quantitative variations in a mitochondrial plasmid-like DNA have been observed. Our results suggest that the cytoplasmic support for male sterility is located in the chromosomal mitochondrial DNA instead of the plasmid-like DNA.
利用限制酶对五种甘蓝型油菜体细胞杂种线粒体基因组进行了分析，这些杂种在第一次实验中结合了甘蓝型油菜叶绿体和来自萝卜的细胞质雄性不育特性，在第二次实验中结合了抗三嗪的芥菜叶绿体和来自萝卜的细胞质雄性不育特性。酶切图谱表明，这些基因组与亲本不同。在体细胞杂种线粒体 DNA 酶切图谱中出现新的带，证明了体细胞杂种细胞中线粒体的重组。在亲本和体细胞杂种植物中，观察到线粒体质粒样 DNA 的大量定量变化。我们的结果表明，细胞质雄性不育的支持位于染色体线粒体 DNA 中，而不是质粒样 DNA 中。
