Kanno A, Kanzaki H, Kameya T
Institute of Genetic Ecology, Tohoku University, 980-77, Sendai, Japan.
Iwate Biotechnology Institute, 024, Kitakami, Iwate, Japan.
Plant Cell Rep. 1997 Apr;16(7):479-484. doi: 10.1007/BF01092770.
In a previous report, intergeneric somatic hybrids between red cabbage (Brassica oleracea L. var.capitata) and radish (Raphanus sativus L. cv. Shougoin) were produced by protoplast fusion. Plant morphology, chromosome number, isozyme patterns, andSma1 cleavage pattern of chloroplast DNA indicated that the hybrid plants have the red cabbage nucleus and the radish chloroplasts. In this report, we analyzed the organization of chloroplast and mitochondrial DNAs from this hybrid using Southern hybridization. The restriction patterns of almost all regions of the chloroplast DNA from the hybrid were similar to that of radish, except for one region near therps16 gene, which encodes the chloroplast ribosomal protein S16. In contrast to chloroplast DNA, the restriction pattern of mitochondrial DNA from the hybrid was quite different from that of the parents.
在之前的一份报告中，通过原生质体融合培育出了红甘蓝（Brassica oleracea L. var.capitata）和萝卜（Raphanus sativus L. cv. Shougoin）之间的属间体细胞杂种。植株形态、染色体数目、同工酶模式以及叶绿体DNA的SmaI切割模式表明，杂种植物具有红甘蓝的细胞核和萝卜的叶绿体。在本报告中，我们利用Southern杂交分析了该杂种的叶绿体和线粒体DNA的组成。杂种叶绿体DNA几乎所有区域的限制性酶切图谱与萝卜的相似，但编码叶绿体核糖体蛋白S16的rps16基因附近的一个区域除外。与叶绿体DNA不同，杂种线粒体DNA的限制性酶切图谱与亲本的差异很大。
