Department of Plant Biology, Allelix Inc., 6850 Goreway Drive, L4V 1P1, Mississauga, Ontario, Canada.
Theor Appl Genet. 1986 Sep;72(6):787-93. doi: 10.1007/BF00266546.
Thirty somatic hybrids between Solanum tuberosum and Solanum brevidens were analysed for mitochondrial and chloroplast genome rearrangements. In all cases, the chloroplast genomes were inherited from one of the parental protoplast populations. No chloroplast DNA alterations were evident but a range of mitochondrial DNA alterations, from zero to extensive intra- and inter-molecular recombinations, were found. Such recombinations involved specific 'recombination hot spots' in the mitochondrial genome. Not all hybrids regenerated from a common callus possessed identical mitochondrial genomes, suggesting that sorting out of mitochondrial populations in the callus may have been incomplete at the plant regeneration stage. Sorting out of organelles in planta was not observed.
对 30 个马铃薯和短柄茄的体细胞杂种进行了线粒体和叶绿体基因组重排分析。在所有情况下，叶绿体基因组都来自一个亲本原生质体群体。虽然没有明显的叶绿体 DNA 改变，但发现了一系列线粒体 DNA 改变，从零到广泛的分子内和分子间重组。这些重组涉及线粒体基因组中的特定“重组热点”。并非所有从共同愈伤组织再生的杂种都具有相同的线粒体基因组，这表明在植物再生阶段，愈伤组织中线粒体群体的分拣可能不完全。未观察到细胞器在植物体内的分拣。
