Agricultural Research Department, Risø National Laboratory, DK-4000, Roskilde, Denmark.
Theor Appl Genet. 1985 Jul;69(4):409-21. doi: 10.1007/BF00570911.
Samples of approximately 100 plants from each of 22 populations ofLolium perenne representing 15 cultivars, and from 13 populations ofLolium multiflorum representing six cultivars were scored for iso-zyme variants in five enzyme systems, PGI, GOT, ACP, PGM and 6-PGD. From the individual banding patterns a genetic interpretation of the variation was formulated and population studies of the resulting six polymorphic enzyme loci were performed. No strong indications of partial selfing was found since at four of the six loci,Pgi 2, Got 3, Pgm 1 andPgd 1, the genotypic proportions were in correspondence with the Hardy-Weinberg expectations. This indicated, further, that the genetical interpretations of the banding patterns might be correct. Deviations from Hardy-Weinberg proportions forAcp 1 andGot 2 indicated presumably selection working on the linkage group including these loci. Gametic phase disequilibrium was observed betweenPgi 2 andPgd 1 for populations of one cultivar. These results were discussed in relation to the variation expected within a cultivar.
从代表 15 个品种的 22 个Lolium perenne种群中，每个种群约取 100 株植物样本；从代表 6 个品种的 13 个Lolium multiflorum种群中，每个种群约取 100 株植物样本，对其进行五个酶系统（PGI、GOT、ACP、PGM 和 6-PGD）的同工酶变体分析。根据个体带型模式，对变异进行了遗传解释，并对由此产生的六个多态性酶基因座的种群研究进行了分析。由于在六个基因座中的四个（Pgi 2、Got 3、Pgm 1 和 Pgd 1），基因型比例与哈迪-温伯格期望相符，因此没有发现部分自交的强烈迹象。这进一步表明，带型模式的遗传解释可能是正确的。Acp 1 和 Got 2 与 Hardy-Weinberg 比例的偏离表明，包括这些基因座在内的连锁群可能存在选择作用。在一个品种的种群中，观察到 Pgi 2 和 Pgd 1 之间的配子相位不平衡。这些结果与品种内预期的变异有关。
