Center for Reproduction of Endangered Species, Zoological Society of San Diego, P.O. Box 551, 92112, San Diego, California.
J Chem Ecol. 1992 May;18(5):703-12. doi: 10.1007/BF00994608.
Femoral gland secretions were collected from 21 captive adult male green iguanas (Iguana iguana) in Orotina, Costa Rica, and San Diego, California, during the breeding (November) and nonbreeding (March) seasons. Lipids were extracted with methylene chloride, weighed, separated by thin-layer chromatography, and analyzed by gas chromatography-mass spectrometry. Free and esterified C14-C26 fatty acids, 5β-cholestan-3α-ol (epico-prostanol), cholest-5-en-3β-ol (cholesterol), cholest-5-en-24-methyl-3β-ol (campesterol), cholesta-5,22-dien-24b-ethyl-3β-ol (stigmasterol), cholesta-8,24(5α)-dien-4,4,14α-trimethyl-3β-ol (lanosterol), cholest-5-en-24-ethyl-3β-ol (β-sitosterol), and two uncharacterized sterols were indicated. More lipids were recovered from femoral gland secretions obtained during the breeding than the nonbreeding months, indicating that secretion deposits may be more detectable during the mating season.
从哥斯达黎加奥罗蒂纳和加利福尼亚圣地亚哥的 21 只圈养成年雄性绿鬣蜥（Iguana iguana）的股腺分泌物中收集到脂质，这些分泌物是在繁殖（11 月）和非繁殖（3 月）季节采集的。用二氯甲烷提取脂质，称重，通过薄层层析分离，并通过气相色谱-质谱分析。游离和酯化的 C14-C26 脂肪酸、5β-胆甾烷-3α-醇（epico-prostanol）、胆甾-5-烯-3β-醇（胆固醇）、胆甾-5-烯-24-甲基-3β-醇（菜油固醇）、胆甾-5,22-二烯-24b-乙基-3β-醇（豆固醇）、胆甾-8,24(5α)-二烯-4,4,14α-三甲基-3β-醇（羊毛固醇）、胆甾-5-烯-24-乙基-3β-醇（β-谷甾醇）和两种未鉴定的甾醇。在繁殖期间从股腺分泌物中回收的脂质多于非繁殖月份，表明在交配季节可能更能检测到分泌物的沉积。
